کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5531031 1549445 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cryopreservation of adult cervid testes
ترجمه فارسی عنوان
پرورش گاوهای شکم بالغ
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


- Testis tissues from three species of adult cervids were cryopreserved using slow freezing protocol in DMSO or DMSO and FBS.
- Cryopreserved-thawed testicular tissues were analyzed for the expression of sperm- and spermatid-specific proteins.
- Retrieved sperm was analyzed for viability, acrosome integrity, morphological preservation, and apoptosis.
- 20% DMSO was acceptable for cryopreservation of barking deer and 20% DMSO with 20% FBS for sambar and hog deer testes.
- The study encourages cryobanking of testis of cervids and provides a basis to develop field-friendly USF protocols.

Several species of cervids are currently classified as threatened or endangered due to a rapid decline in their populations. Sperm cryopreservation, in association with assisted reproductive technologies, can find application for the conservation of endangered cervids. In cases of unsuccessful sperm retrieval through other means prior to the death of the animal, adult testis is the only source of sperm. Recovery of viable sperm from adult testes depends on the effective preservation of testicular tissues through optimization of cryopreservation protocols. The present study evaluated combinations of 10% dimethyl sulfoxide (DMSO) with 0% or 80% fetal bovine serum (FBS) and 20% DMSO with 0 or 20% FBS for the cryopreservation of testicular tissues of three adult cervids using uncontrolled slow freezing protocol. The cryopreserved testis was compared to chilled tissue without cryoprotectants. Results revealed that testicular tissues of barking deer cryopreserved in 20% DMSO (D20) had all the analyzed 7 parameters (number of TNP1-, PRM2 and acrosin-expressing cells/tubule and, the number of viable, morphologically normal, acrosome intact, Annexin V-negative sperm) comparable to the chilled testis. However, testicular tissues of sambhar and hog deer cryopreserved only in D20S20 had 5 of 7 parameters comparable to the chilled testis. In conclusion, D20 is acceptable for cryopreservation of barking deer and D20S20 for sambar and hog deer testes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 74, February 2017, Pages 103-109
نویسندگان
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