کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5531997 1401825 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Role of CD34 family members in lumen formation in the developing kidney
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Role of CD34 family members in lumen formation in the developing kidney
چکیده انگلیسی


- Endoglycan (EG) is a novel pre-luminal marker of renal and pancreas epithelial tubules.
- EG localizes to Rab11+ vesicles, then relocalizes to the forming apical surface.
- Our data suggest renal lumens form via vesicular trafficking of apical cargo.
- Circular structures that may be exosomes are present in renal epithelial lumens.
- CD34 family members are dispensable for continuous lumen formation in the kidney.

Previous studies have shown CD34 family member Podocalyxin is required for epithelial lumen formation in vitro. We demonstrate that Endoglycan, a CD34 family member with homology to Podocalyxin, is produced prior to lumen formation in developing nephrons. Endoglycan localizes to Rab11-containing vesicles in nephron progenitors, and then relocalizes to the apical surface as progenitors epithelialize. Once an apical/luminal surface is formed, Endoglycan (and the actin-binding protein Ezrin) localize to large, intraluminal structures that may be vesicles/exosomes. We generated mice lacking Endoglycan and found mutants had timely initiation of lumen formation and continuous lumens, similar to controls. Mice with conditional deletion of both Endoglycan and Podocalyxin in developing nephrons also had normal tubular lumens. Despite this, Endoglycan/Podocalyxin is required for apical recruitment of the adaptor protein NHERF1, but not Ezrin, in podocyte precursors, a subset of the epithelia. In summary, while CD34 family members appear dispensable for lumen formation, our data identify Endoglycan as a novel pre-luminal marker and suggest lumen formation occurs via vesicular trafficking of apical cargo that includes Endoglycan.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 418, Issue 1, 1 October 2016, Pages 66-74
نویسندگان
, , , , , , , , ,