کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5532847 1402082 2017 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
ReviewSensing Membrane Curvature in Macroautophagy
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
ReviewSensing Membrane Curvature in Macroautophagy
چکیده انگلیسی


- Autophagosome biogenesis involves significant structural remodeling of cellular membranes.
- Proteins target these unique morphologies via curvature-sensing motifs.
- Each of the major autophagy complexes utilizes one or more curvature-sensitive proteins.

In response to intracellular stress events ranging from starvation to pathogen invasion, the cell activates one or more forms of macroautophagy. The key event in these related pathways is the de novo formation of a new organelle called the autophagosome, which either surrounds and sequesters random portions of the cytoplasm or selectively targets individual intracellular challenges. Thus, the autophagosome is a flexible membrane platform with dimensions that ultimately depend upon the target cargo. The intermediate membrane, termed the phagophore or isolation membrane, is a cup-like structure with a clear concave face and a highly curved rim. The phagophore is largely devoid of integral membrane proteins; thus, its shape and size are governed by peripherally associated membrane proteins and possibly by the lipid composition of the membrane itself. Growth along the phagophore rim marks the progress of both organelle expansion and ultimately organelle closure around a particular cargo. These two properties, a reliance on peripheral membrane proteins and a structurally distinct membrane architecture, suggest that the ability to target or manipulate membrane curvature might be an essential activity of proteins functioning in this pathway. In this review, we discuss the extent to which membranes are naturally curved at each of the cellular sites believed to engage in autophagosome formation, review basic mechanisms used to sense this curvature, and then summarize the existing literature concerning which autophagy proteins are capable of curvature recognition.

Graphical AbstractMembrane curvature on the growing autophagosome. Subdomains of the growing autophagosome present unique membrane architectures that likely recruit or activate subsets of macroautophagy proteins.37

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Biology - Volume 429, Issue 4, 17 February 2017, Pages 457-472
نویسندگان
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