کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5534481 1402181 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Original research articleFour novel polymorphisms of buffalo INSIG2 gene are associated with milk production traits in Chinese buffaloes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Original research articleFour novel polymorphisms of buffalo INSIG2 gene are associated with milk production traits in Chinese buffaloes
چکیده انگلیسی


- Four SNPs of INSIG2 were detected in a total of 264 Chinese buffalo individuals.
- Four SNPs had significantly associated with 305-day milk yield or protein percentage in Murrah and Crossbred breeds.
- The diplotype H1H1 showed significantly associated with 305-day milk yield in Murrah buffaloes.
- Buffalo INSIG2 gene could be used as a candidate gene for buffalo breeding program.

Insulin-induced genes (INSIGs), including INSIG1 and INSIG2, are important mediators that play a pivotal role in the lipid metabolism and could cause the retention of the SCAP/SREBP complex. Therefore, the objective of this study is to detect the single nucleotide polymorphisms (SNPs) of buffalo INSIG2 gene and evaluate their associations with milk production traits in Chinese buffaloes. A total of four SNPs (g.621272A > G, g.621364A > C, g.632543G > A, and g.632684C > T) were identified using DNA pooled sequencing, and the SNP genotyping for the identified SNPs was performed by using Matrix-assisted laser desorption/ionization time of flight mass spectrometry method from 264 individuals. The results showed that four SNPs were significantly associated with 305-day milk yield or protein percentage in Murrah and crossbred breeds (P < 0.05), but they had no significant effect on milk production traits in Nili-Ravi buffaloes (P > 0.05). Linkage disequilibrium (LD) analysis revealed that one haplotype block was successfully constructed, of which the diplotype H1H1 showed significant association with 305-day milk yield in Murrah buffaloes (P < 0.05). Our findings provide evidence that polymorphisms in buffalo INSIG2 gene are associated with milk production traits, and could be used as a candidate gene for marker-assisted selection in buffalo breeding program.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Probes - Volume 30, Issue 5, October 2016, Pages 294-299
نویسندگان
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