| کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
|---|---|---|---|---|
| 5534495 | 1551171 | 2017 | 5 صفحه PDF | دانلود رایگان |
کلمات کلیدی
1.مقدمه
2. مواد و روش ها
2.1 معرف ها و مواد
2.2 اندازه گیری میزان نور فلورسانت
2.3 بررسی فعالیت DNA پلی مراز در لحظه
2.4 بهینه سازی شرایط آنالیز
2.5 ارزیابی فعالیت DNA پلی مراز
2.6 تاثیر مهار کنندگان بر فعالیت DNA پلی مراز
3. نتایج و بحث
3.1 پایه و اساس استراتژی
3.2 بررسی فعالیت DNA پلی مراز در لحظه
3.3 بهینه سازی شرایط آزمایشات
3.4 کمیت فعالیت DNA پلی مراز
3.5 تاثیر مهار کنندگان بر فعالیت DNA پلی مراز
4.نتیجه گیری
اشکال و جداول
- A novel, real-time fluorescence assay has been developed for the detection of DNA polymerase activity.
- This method is highly sensitive with a detection limit of 0.1 U/mL.
- This method has been applied to detect DNA polymerase inhibitor.
- It is simple and cost-effective without requirement of labeling with a fluorophore-quencher pair.
We have developed a label-free assay for the detection of DNA polymerase activity based on a thrombin-binding aptamer (TBA) G-quadruplex. In the presence of DNA polymerase, the 3â²-OH termini of the hairpin substrate are immediately elongated to replace the TBA, which can be recognized quickly by the ThT dye and results in an increase of fluorescence. This method is highly sensitive with a detection limit of 0.1 U/mL. It is simple and cost-effective without any requirement of labeling with a fluorophore-quencher pair. Furthermore, the proposed method can also be applied to analyze the inhibition of DNA polymerase, which clearly indicates that the proposed method can be applied for screening of potential DNA polymerase inhibitors.
Journal: Molecular and Cellular Probes - Volume 32, April 2017, Pages 13-17