Short communicationDevelopment of a real-time PCR for Bartonella spp. detection, a current emerging microorganism

- A real-time PCR assay to detect Bartonella spp. species has been developed.
- This assay has been tested with the most frequent species of Bartonella spp.
- Its flexibility may allow to detect more species, even those not described yet.

A real-time PCR assay using SYBR Green was optimized to detect those Bartonella that are most frequently described as pathogens. The assay was genus-specific. Sequencing allowed to distinguish species. Assay sensitivity was determined using 10-fold serial dilutions of genomic DNA. Dynamic range was 100 ng-100 fg and sensitivity was 50 copies/reaction.