کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5534501 | 1551171 | 2017 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Short communicationDevelopment of a real-time PCR for Bartonella spp. detection, a current emerging microorganism
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
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چکیده انگلیسی
- A real-time PCR assay to detect Bartonella spp. species has been developed.
- This assay has been tested with the most frequent species of Bartonella spp.
- Its flexibility may allow to detect more species, even those not described yet.
A real-time PCR assay using SYBR Green was optimized to detect those Bartonella that are most frequently described as pathogens. The assay was genus-specific. Sequencing allowed to distinguish species. Assay sensitivity was determined using 10-fold serial dilutions of genomic DNA. Dynamic range was 100Â ng-100Â fg and sensitivity was 50 copies/reaction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Probes - Volume 32, April 2017, Pages 55-59
Journal: Molecular and Cellular Probes - Volume 32, April 2017, Pages 55-59
نویسندگان
Elena Parra, Ferran Segura, Jessica Tijero, Imma Pons, Maria-Mercedes Nogueras,