کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5539878 1553515 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Transcutaneous immunization of Streptococcus suis bacterin using dissolving microneedles
ترجمه فارسی عنوان
ایمن سازی پوستی باکتری استرپتوکوک سویس با استفاده از میکروسکوپ حل کننده
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی


- Dissolving microneedle vaccine containing S. suis 2 bacterin was evaluated in mice.
- Microneedles induce TH1-type cytokine production, antibody response, and strong T cell proliferation.
- Single-dose microneedle vaccination provides higher protection rate in challenge test than intramuscular vaccination.
- Single-dose microneedle vaccination maintains TH1-type antibody levels for up to 7 months.

Vaccine delivery using microneedle (MN) patches is an easy, safe and painless alternative to traditional needle injections. In this study, we examined whether MN patches can enhance the efficacy of a Streptococcus suis serotype 2 (S. suis 2) vaccine in a mouse model. Results showed that MNs can reach 200-250 μm into the skin, a depth beneficial for targeted delivery of antigens to antigen-presenting cells in the epidermis and dermis. Vaccination with prime-boost of MN induced higher levels of IgG2a antibody titer, T cell proliferation, and TH1 cytokines (IFN-γ and IL-12) as compared to intramuscular (IM) injection. In addition, single dose MN vaccination better protected mice against lethal challenge than IM vaccination. MN vaccination also conferred long-term IgG2a antibody against S. suis 2 bacteria presence for up to 7 months. Taken together, these data showed that vaccine delivery by MNs results in superior immune response and protection rate when compared to IM injections.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Immunology, Microbiology and Infectious Diseases - Volume 50, February 2017, Pages 78-87
نویسندگان
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