کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5540435 | 1553613 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification and activity of a paralog of cathepsin S from yellow catfish (Pelteobagrus fulvidraco) involved in immune response
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
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چکیده انگلیسی
Cathepsin S, a papain-like cysteine peptidase, is an important regulator and signaling molecule with diverse biological actions in addition to immune presentation. However, our understanding of its structure and properties remains limited. Herein, a full-length cathepsin Sa from yellow catfish was cloned and named PfCTSSa. It contained 1366 bp, including a 981 bp ORF flanked by a 123 bp 5â²-untranslated region (UTR) and a 262 bp 3â²-UTR. This ORF encoded a 36.5 kD cysteine protease with the deduced amino acid sequence having a 76% sequence identity with Ictalurus punctatus ctssa. Additionally, PfCTSSa was found to be a paralog of cathepsin S since it generated a new cluster with cathepsin Sa in the phylogenic tree. Furthermore, PfCTSSa was found to contain more N-glycosylation sites than cathepsin S. The recombinant PfCTSSa was overexpressed in E. coli BL21 (DE3) and appeared to have the strongest activity at pH 8.5 and 35 °C in a concentration-dependent manner, with activity further affected by metal ions and detergents. Moreover, PfCTSSa mRNA was highly expressed in classic and mucosal immune tissues, although constitutively distributed in all of the examined tissues. Yellow catfish were then challenged with inactivated Aeromonas hydrophila and PfCTSSa was remarkably increased in the head kidney, liver and spleen when compared to the PBS control. Collectively, these results indicate that PfCTSSa is a paralog of cathepsin S and functions in the yellow catfish immune response.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 61, February 2017, Pages 16-23
Journal: Fish & Shellfish Immunology - Volume 61, February 2017, Pages 16-23
نویسندگان
Yun Wang, Xin Liu, Shuai Lv, Jinnan Ren, Fei Ke,