کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5540469 1553609 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of PmSpӓtzle 1 from the black tiger shrimp Peneaus monodon
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Characterization of PmSpӓtzle 1 from the black tiger shrimp Peneaus monodon
چکیده انگلیسی
Spätzle is a signaling ligand in innate immune response that signals pathogenic infection via Toll receptor and Toll pathway into the cells for the synthesis of antimicrobial proteins. Herein, three PmSpӓtzle isoforms were identified in Penaeus monodon, namely PmSpz1, 2 and 3. The PmSpz1 was chosen for detailed study. The PmSpz1 gene was expressed in all nine tissues tested including the hemocytes, stomach, hepatopancreas, gill, lymphoid tissue, eyestalk, muscle, intestine and heart. Its expression was up-regulated upon white spot syndrome virus (WSSV) infection. Western blot analysis of hemolymph showed that the PmSpz1 mostly existed as a cleaved active form awaiting to activate the Toll pathway. Injection of a recombinant PmSpz1 rendered the shrimp less susceptible to the WSSV infection. Injection of a recombinant active form of PmSpz1 into a normal shrimp activated the synthesis of crustinPm1, crustinPm7, ALFPm3, penaeidin3 but not penaeidin5 indicating that the expression of all antimicrobial proteins but not penaeidin5 was under the regulation of Toll pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 65, June 2017, Pages 88-95
نویسندگان
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