کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5540662 1553616 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of 10 transcripts of FREP in penaeid shrimp Litopenaeus vannamei
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Identification of 10 transcripts of FREP in penaeid shrimp Litopenaeus vannamei
چکیده انگلیسی


- Totally 10 transcripts were identified and characterized in penaeid shrimp Litopenaeus vannamei.
- The tissue distribution and expression post bacteria and virus challenge were analyzed.
- Recombinant LvFREP could agglutinate bacteria Vibrio harveyi in the presence of Ca2+.

Fibrinogen-related proteins (FREPs) are widely distributed in vertebrates and invertebrates and known having Fibrinogen-related domains (FReDs) which function in multiple aspects, especially in innate immune response as pattern recognition receptors. However, there is no any report about FREP in penaeid shrimp Litopenaeus vannamei. Here, totally 10 transcripts of FREP were isolated and named LvFREP1.1, 1.2 until 1.10. All of the 10 transcripts have high identity in their 3′ ends and encode conserved FReDs. Since the 10 transcripts are highly similar we could not design any primers that can amplify a single transcript. We chose a pair of primers corresponding to part of LvFREP1.1 and LvFREP1.5 to examine their expression. Tissue distribution indicated LvFREP1.1 and LvFREP1.5 mRNA locates in hemocytes, gills, intestine, hearts and slightly in nerve. The expression of LvFREP1.1 and LvFREP1.5 behaves differently post bacteria and virus infection. Besides, recombinant LvFReD could agglutinate bacteria Vibrio harveyi in the presence of Ca2+. These initial data presents the diversity of FREPs in penaeid shrimp and also push us to further explore their roles in shrimp immune response.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 58, November 2016, Pages 436-441
نویسندگان
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