کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5541656 | 1553846 | 2017 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Potential factors involved in virulence of Cronobacter sakazakii isolates by comparative transcriptome analysis
ترجمه فارسی عنوان
عوامل پتانسیل درگیر شدن در جدایه های کرونوباکتر ساکازاکی با استفاده از تجزیه و تحلیل ترانس کریتومتر مقایسه شده است
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کلمات کلیدی
کرونوباکتر، تجزیه و تحلیل عامل ویروسی پرچم پروتئین غشای خارجی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
Cronobacter species are important foodborne pathogens causing severe infections in neonates through consumption of contaminated powdered infant formula. However, the virulence-associated factors in Cronobacter are largely unknown. In this study, the transcriptome analysis between highly virulent Cronobacter sakazakii G362 and attenuated L3101 strains was used to reveal the potential factors involved in virulence. The total transcripts were grouped into 20 clusters of orthologous group categories and summarized in 3 gene ontology categories (biological process, cellular component, and molecular function). In addition, the differentially expressed genes (DEG) between these isolates were analyzed using Volcano plots and gene ontology enrichment. The predominant DEG were flagella-associated genes such as flhD, motA, flgM, flgB, and fliC. Furthermore, the expression abundance of outer membrane protein or lipoprotein genes (ompW, slyB, blc, tolC, and lolA), potential virulence-related factors (hlyIII and hha), and regulation factors (sdiA, cheY, Bss, fliZ) was also significantly different between G362 and L3101. Interestingly, 3 hypothetical protein genes (ESA_01022, ESA_01609, and ESA_00609) were found to be expressed only in G362. Our findings provide valuable transcriptomic information about potential virulence factor genes, which will be needed in future molecular biology studies designed to understand the pathogenic mechanism of Cronobacter.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dairy Science - Volume 100, Issue 11, November 2017, Pages 8826-8837
Journal: Journal of Dairy Science - Volume 100, Issue 11, November 2017, Pages 8826-8837
نویسندگان
Yingwang Ye, Xiyan Zhang, Maofeng Zhang, Na Ling, Haiyan Zeng, Jina Gao, Rui Jiao, Qingping Wu, Jumei Zhang,