کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5542669 1402521 2016 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rumen-protected methionine compared with rumen-protected choline improves immunometabolic status in dairy cows during the peripartal period
ترجمه فارسی عنوان
متیونین محافظت شده با رامن در مقایسه با کولین محافظت شده با شکمبه باعث بهبود وضعیت ایمنی مکانیکی در گاوهای شیری در طی دوره پریاپارتال
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی
The immunometabolic status of peripartal cows is altered due to changes in liver function, inflammation, and oxidative stress. Nutritional management during this physiological state can affect the biological components of immunometabolism. The objectives of this study were to measure concentrations of biomarkers in plasma, liver tissue, and milk, and also polymorphonuclear leukocyte function to assess the immunometabolic status of cows supplemented with rumen-protected methionine (Met) or choline (CHOL). Forty-eight multiparous Holstein cows were used in a randomized complete block design with 2 × 2 factorial arrangement of Met (Smartamine M, Adisseo NA, Alpharetta, GA) and CHOL (ReaShure, Balchem Inc., New Hampton, NY) level (with or without). Treatments (12 cows each) were control (CON), no Met or CHOL; CON and Met (SMA); CON and CHOL (REA); and CON and Met and CHOL (MIX). From −50 to −21 d before expected calving, all cows received the same diet [1.40 Mcal of net energy for lactation (NEL)/kg of DM] with no Met or CHOL. From −21 d to calving, cows received the same close-up diet (1.52 Mcal of NEL/kg of DM) and were assigned randomly to each treatment. From calving to 30 d, cows were on the same postpartal diet (1.71 Mcal of NEL/kg of DM) and continued to receive the same treatments until 30 d. The Met supplementation was adjusted daily at 0.08% DM of diet, and CHOL was supplemented at 60 g/cow per day. Liver (−10, 7, 21, and 30 d) and blood (−10, 4, 8, 20, and 30 d) samples were harvested for biomarker analyses. Neutrophil and monocyte phagocytosis and oxidative burst were assessed at d 1, 4, 14, and 28 d. The Met-supplemented cows tended to have greater plasma paraoxonase. Greater plasma albumin and IL-6 as well as a tendency for lower haptoglobin were detected in Met- but not CHOL-supplemented cows. Similarly, cows fed Met compared with CHOL had greater concentrations of total and reduced glutathione (a potent intracellular antioxidant) in liver tissue. Upon a pathogen challenge in vitro, blood polymorphonuclear leukocyte phagocytosis capacity and oxidative burst activity were greater in Met-supplemented cows. Overall, liver and blood biomarker analyses revealed favorable changes in liver function, inflammation status, and immune response in Met-supplemented cows.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dairy Science - Volume 99, Issue 11, November 2016, Pages 8956-8969
نویسندگان
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