کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5543830 | 1554296 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization and biological activity of bovine interferon-omega3
ترجمه فارسی عنوان
خصوصیات مولکولی و فعالیت بیولوژیکی اینترفرون گاو-امگا 3
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
Bovine interferon-omega3 (BoIFN-Ï3) gene was amplified from bovine liver genomic DNA, which encodes a 195-amino acid protein containing a 23-amino acid signal peptide. Analysis of the molecular characteristics revealed that BoIFN-Ï3 evolving from IFN-Ï, contained four cysteine residues and five alpha helices, showing that BoIFN-Ï3 presented the typical molecular characteristics of type I interferon. BoIFN-Ï3 exhibited antiviral and antiproliferative activities, which exerted a protective effect against VSV in several mammalian cell lines, as well as against BEV, IBRV, and BVDV in MDBK cell. Moreover, BoIFN-Ï3 was shown to be highly sensitive to trypsin, but remaining stable despite changes in pH and temperature. Additionally, BoIFN-Ï3 induced the transcription of Mx1, ISG15, and ISG56 genes, as well as the expression of Mx1 protein in a time-dependent manner. These findings will be useful to further study BoIFN-Ï in host's defence against infectious diseases, particularly viral infections. Furthermore, results will facilitate further research on the bovine interferon family.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Veterinary Science - Volume 115, December 2017, Pages 125-131
Journal: Research in Veterinary Science - Volume 115, December 2017, Pages 125-131
نویسندگان
Dong An, Yongli Guo, Jun Bao, Xiuxin Luo, Ying Liu, Bo Ma, Mingchun Gao, Junwei Wang,