کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5551151 | 1402940 | 2017 | 8 صفحه PDF | دانلود رایگان |

Rabdosia rubescens is a healthy herbal tea and well-known Chinese medicinal herb. To evaluate the quality of R. rubescens from China, a high performance liquid chromatography method with dual-wavelength detection was developed and validated. The method was successfully applied for the simultaneous characterization and quantification of 17 main constituents from four different cultivation regions in China. Under optimal conditions, analysis was performed on a Luna C-18 column and gradient elution with a solvent system of acetonitrile and 0.5% (v/v) acetic acid-water at a flow rate of 1.0Â mL/min and wavelength of 220Â nm and 280Â nm. All standard calibration curves exhibited good linearity (r2Â >Â 0.9992) within the test ranges. The precision was evaluated by intraday and interday tests, which revealed relative standard deviation values within the ranges of 0.57-2.35% and 0.52-3.40%, respectively. The recoveries were in the range of 96.37-101.66%. The relative standard deviation values for stability and repeatability were < 5%. The contents of some compounds were low and varied with different cultivars. The proposed method could serve as a prerequisite for quality control of R. rubescens materials and products.
296A novel rapid high-performance liquid chromatography method was established for the simultaneous quantification of 17Â compounds from four kinds of Rabdosia rubescens cultivated from different regions in China.
Journal: Journal of Food and Drug Analysis - Volume 25, Issue 2, April 2017, Pages 417-424