کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5559846 1561691 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Connexin 43 mediates changes in protein phosphorylation in HK-2 cells during chronic cadmium exposure
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Connexin 43 mediates changes in protein phosphorylation in HK-2 cells during chronic cadmium exposure
چکیده انگلیسی
Connexin 43 (Cx43) is believed to play a role in the mechanisms of toxicity of many chemical species, include cadmium (Cd). In this study, human renal proximal tubule (HK-2) cells were exposed to Cd (1 μM, 10 days). Of the 584 protein residues detected using a Phospho Explorer antibody microarray (PEX100), more than half changed their levels of phosphorylation after chronic Cd exposure. Cx43 siRNA attenuated Cd-induced apoptosis and inhibited proliferation, while also attenuating changes in the levels of phosphorylation of many protein residues. According to DAVID Bioinformatics Resources analysis and KEGG PATHWAY database, AKT signal pathway may be the important one. Focusing on the AKT pathway confirmed that Cx43 mediated increased levels of p-PTENSer380/Ser382/Thr383 and decreased levels of p-AKTThr308, p-AKTTyr326, p-ASK1Ser83, and p-p27Thr187, thereby possibly contributing to the Cd-induced apoptosis and inhibited proliferation. These results suggested that AKT pathway was the dominant pathway involved in Cx43-mediated chronic Cd toxicity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Toxicology and Pharmacology - Volume 53, July 2017, Pages 184-190
نویسندگان
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