Polarized monolayer cultures of human intestinal epithelial cell lines exposed to intractable proteins - In vitro hazard identification studies

- Intractable proteins did not produce adverse effects toward human intestinal epithelial cell (IEC) monolayers.
- Intractable proteins do not appear to be inherently hazardous as a class of proteins.
- In vitro methods of protein hazard identification require much less protein than studies with laboratory animals.
- Inexpensive and readily available cells, reagents, and instruments can be used for protein hazard identification.