کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5561892 | 1562295 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mitochondrial functions of THP-1 monocytes following the exposure to selected natural compounds
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کلمات کلیدی
IC10CBD3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromideIC50DeoxynivalenolFCCPRSCOCRMTT - MTTCSA - ایالات مؤتلفهٔ آمریکاMitochondrial functions - توابع میتوکندریاییDON - دونelectron transport chain - زنجیره انتقال الکترونTHP-1 cells - سلول های THP-1Immunotoxicity - سمیت ایمنیCyclosporine A - سیکلوسپورینAinhibitory concentration 50 - غلظت مهاری 50Oxygen consumption rate - میزان مصرف اکسیژنETc - و غیرهCannabidiol - کانابیدیول
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The immune system is an important target of various xenobiotics, which may lead to severe adverse effects including immunosuppression or inappropriate immunostimulation. Mitochondrial toxicity is one possibility by which xenobiotics exert their toxic effects in cells or organs. In this study, we investigated the impact of three natural compounds, cyclosporine A (CsA), deoxynivalenol (DON) and cannabidiol (CBD) on mitochondrial functions in the THP-1 monocytic cell line. The cells were exposed for 24Â h to two different concentrations (IC10 and IC50 determined by MTT) of each compound. The cells showed concentration-dependent elevated intracellular reactive oxygen species (iROS) and induction of apoptosis (except DON) in response to the three test compounds. Mitochondrial functions were characterized by using bioenergetics profiling experiments. In THP-1 monocytes, the IC50 of CsA decreased basal and maximal respiration as well as ATP production with an impact on spare capacity indicating a mitochondrial dysfunction. Similar reaction patterns were observed following CBD exposure. The basal respiration level and ATP-production decreased in the THP-1 cells exposed to the IC50 of DON with no major impact on mitochondrial function. In conclusion, impaired mitochondrial function was accompanied by elevated iROS and apoptosis level in a monocytic cell line exposed to CsA and CBD. Mitochondrial dysfunction may be one explanation for the cytotoxicity of CBD and CsA also in other in immune cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 377, 15 February 2017, Pages 57-63
Journal: Toxicology - Volume 377, 15 February 2017, Pages 57-63
نویسندگان
Nadin Schultze, Heike Wanka, Paula Zwicker, Ulrike Lindequist, Beate Haertel,