کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5584391 | 1404307 | 2016 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Responses of adventitial CD34+ vascular wall-resident stem/progenitor cells and medial smooth muscle cells to carotid injury in rats
ترجمه فارسی عنوان
واکنش سلول های ساقه / پروتروه سلولی ساطع کننده دیواره دیابتی عروقی و سلول های عضلانی مدیال به آسیب های کاروتید در موش صحرایی
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کلمات کلیدی
DAPI5-bromo-2-deoxyuridineSMCsPlatelet-derived growth factor BB4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولBrdU - بروموداکسی اوریدینProliferation - ترویجSmooth muscle myosin heavy chain - زنجیر سنگین میوزین عضله صافVascular smooth muscle cells - سلول های عضلانی صاف عروقیSmooth muscle cells - سلول های عضله صافNeointimal formation - شکل گیری Neointimal
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی بالینی
چکیده انگلیسی
Cell culture and carotid injury studies with SD rats were performed to investigate the roles of CD34+ vascular wall-resident stem/progenitor cells (VRS/Pcs) and vascular smooth muscle cells (SMCs) in neointimal formation. In vitro, the media-isolated SM MHC+ SMCs occupied 93.92 ± 8.62% of total BrdU+ cells, whereas the CD34+ cells, only 2.61 ± 0.82%, indicating that the cell expansion in SMC culture was attributed to SM MHC+ SMCs. The adventitia-isolated CD34+ VRS/Pcs responded to PDGF-BB by differentiating into SMC-like cells which expressed SM22α (an early stage SMC marker), but seldom SM MHC (a late stage SMC marker). In carotid injury model, the CD34+ VRS/Pcs differentiated SMC-like cells migrated in very few numbers into only the outer layer of the media, and this was further confirmed by a cell tracking analysis. While the neointimal cells were consistently SM MHC+ and CD34â SMCs during whole course of the post-injury remodeling. Thus it is speculated that the adventitial CD34+ VRS/Pcs, at least in rat model, do not directly participate in neointimal formation, but function to maintain homeostasis of the media during injury-induced vascular wall remodeling.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental and Molecular Pathology - Volume 101, Issue 3, December 2016, Pages 332-340
Journal: Experimental and Molecular Pathology - Volume 101, Issue 3, December 2016, Pages 332-340
نویسندگان
Yan Shen, Yan Wu, Yong Zheng, Feng Ao, Kai Kang, Yu Wan, Jian Song,