کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5586900 | 1568723 | 2017 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
CK2 induced RIG-I drives metabolic adaptations in IFNγ-treated glioma cells
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کلمات کلیدی
G6PDSfMDCFDACK2IFNγRIG-ITIGARROS - ROSdihydroethidium - دی هیدروتیدیمdichlorodihydrofluorescein diacetate - دی کلستیدوفروفوروزین دی سکتهSerum free media - رسانه آزاد سرمMetabolism - متابولیسم DHE - وCasein kinase 2 - کازئین کیناز 2Interferon gamma - گاما اینترفرونGlucose 6 phosphate dehydrogenase - گلوکز 6 فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
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چکیده انگلیسی
Given the known anti-tumorigenic properties of IFNγ, its effect on glioma cell survival was investigated. Though IFNγ had no effect on glioma cell viability, it induced cell cycle arrest. This was accompanied by increased expression of p53 and retinoic acid inducible gene (RIG-I). While RIG-I had no effect on glioma cell survival, it increased expression of p53 and its downstream target TP53 induced glycolysis and apoptosis regulator (TIGAR). IFNγ induced mitochondrial co-localization of RIG-I was concomitant with its ability to regulate ROS generation, oxidative phosphorylation (OXPHOS) and key enzymes involved in glycolysis and pentose phosphate pathway. Importantly, metabolic gene profiling indicated a suppressed glycolytic pathway in glioma cells upon IFNγ treatment. In addition, IFNγ mediated increase in casein kinase 2 (CK2) expression positively regulated RIG-I expression. These findings demonstrate how IFNγ induced CK2 regulates RIG-I to drive a complex program of metabolic adaptation and redox homeostasis, crucial for determining glioma cell fate.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytokine - Volume 89, January 2017, Pages 219-228
Journal: Cytokine - Volume 89, January 2017, Pages 219-228
نویسندگان
Ruchi Ghildiyal, Ellora Sen,