کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589432 | 1569835 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Minimal essential region for krüppel-like factor 5 expression and the regulation by specificity protein 3-GC box binding
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کلمات کلیدی
LUCKLFRLAshort interfering RNA - RNA تداخل کوتاهsiRNA - siRNAchromatin immunoprecipitation - ایمن سازی کروماتینMER - بیشترTranscription promoter - رونویسی پروموترGene transcription - رونویسی ژنCancer - سرطانTranscription factor - عامل رونویسیKrüppel-like factor - عامل کریپال مانندluciferase - لوسیفرازpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازCHiP - چیپ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
Krüppel-like factor 5 (KLF5) transcriptionally controls the proliferation-differentiation balance of epithelium and is overexpressed in carcinomas. Although genomic region modifying KLF5 expression is widespread in different types of cells, the region that commonly regulates basal expression of the genes across cell-types is uncertain. In this study we determined the minimal essential region for the expression and its regulatory transcription factors using oral carcinoma cells. A reporter assay defined a 186Â bp region downstream of the transcription start site and a cluster of six GC boxes (GC1-GC6) as the minimal essential region. Mutation in the GC1 or GC6 regions but not other GC boxes significantly decreased the reporter expression. The decrease by the GC1 mutation was reproduced in the 2Â kbp full-length promoter, but not by the GC6 mutation. Additionally, specificity proteins (Sp) that can be expressed in epithelial cells and bind GC box, Sp3 co-localized with KLF5 in oral epithelium and carcinomas and chromatin immunoprecipitation analyses showed Sp3 as the prime GC1-binding protein. Inhibition of Sp-GC box binding by mithramycin A and knockdown of Sp3 by the short interfering RNA decreased expression of the reporter gene and endogenous KLF5. These data demonstrate that a 186Â bp region is the minimal essential region and that Sp3-GC1 binding is essential to the basal expression of KLF5.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 601, 15 February 2017, Pages 36-43
Journal: Gene - Volume 601, 15 February 2017, Pages 36-43
نویسندگان
Nozomi Mihara, Tadashige Chiba, Kosuke Yamaguchi, Haruka Sudo, Hisao Yagishita, Kazushi Imai,