کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589549 | 1569803 | 2017 | 36 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
DjhnRNPA2/B1-like gene is required for planarian regeneration and tissue homeostasis
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کلمات کلیدی
qPCRmRNAPlanarianNCBIORFcDNA - cDNAComplementary DNA - DNA تکمیلیhnRNPs - hnRNP هاmessenger RNA - RNA messengerRNA interference - RNA تداخل کنندهRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهWISH - آرزو کردنRegeneration - باززاییReconstruction - بازسازیbase pair - جفت پایهDAY - روزHeterogeneous nuclear ribonucleoproteins - ریبونولوپروتئین های هسته ای ناهمگنopen reading frame - قاب خواندن بازThe National Center for Biotechnology Information - مرکز ملی بیوتکنولوژی اطلاعاتRace - مسابقهUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیwhole-mount in situ hybridization - کلیدی در هیبریداسیون in situ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The hnRNPs play important roles in physiological processes in eukaryotic organisms by regulation of pre-mRNA after transcription, including pre-mRNA splicing, mRNA stability, DNA replication and repair and telomere maintenance and so on. However, it remains unclear about the specific functions of these genes. In this study, the full-length cDNA sequence of hnRNPA2/B1-like was first cloned from Dugesia japonica, and its roles were investigated by WISH and RNAi. The results showed that: (1) DjhnRNPA2/B1-like was highly conserved during animal evolution; (2) DjhnRNPA2/B1-like mRNA was mainly distributed each side of the body in intact worms and regenerative blastemas, and its expression levels were up-regulated on days 0 and 5 after amputation; (3) the intact and regenerating worms gradually lysed or lost regeneration capacity after DjhnRNPA2/B1-like RNAi; and (4) DjhnRNPA2/B1-like expression is induced by temperature and heavy metal ion stress. The data suggests that DjhnRNPA2/B1-like is a multiple functional gene, it plays important roles in regeneration and homeostatic maintenance and it is also involved in stress responses in planarians. Our work provides basic data for the study of regenerative mechanism and stress responses in freshwater planarians.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 633, 30 October 2017, Pages 9-16
Journal: Gene - Volume 633, 30 October 2017, Pages 9-16
نویسندگان
Zimei Dong, Tong Yang, Yibo Yang, He Dou, Guangwen Chen,