کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589566 | 1569811 | 2017 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Alternative polyadenylation expands the mRNA isoform repertoire of human CD46
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کلمات کلیدی
CCP3′RACE5′SSsnRNPpolyadenylation siteCD46reverse transcription - polymerase chain reactionRTKFBSIPANSDMCPRT-PCRRCAASO3′ untranslated region - 3 منطقه غیر ترجمه3′UTR - 3'UTRantisense oligonucleotide - oligonucleotide antisenseAlternative polyadenylation - polyadenylation جایگزینSTP - PTSregulators of complement activation - تنظیم کننده های مکمل فعال سازیRTK, Receptor tyrosine kinase - تیروزین کینازهای گیرنده ایsmall nuclear ribonucleoprotein - ریبونولوپروتئین کوچک هسته ایfetal bovine serum - سرم جنین گاوtransmembrane - فرابنفشUnknown - ناشناسPAS - نهcomplement control protein - پروتئین کنترل مکملmembrane cofactor protein - پروتئین کوفتور غشاءPseudogene - پزوژنSignal peptide - پپتید سیگنالAPA - چی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
Alternative polyadenylation is a prevalent mechanism regulating mammalian gene expression. While tandem 3â²-Untranslated-Region (3â²UTR) polyadenylation changes expression levels, Intronic PolyAdenylation generates shorter transcripts encoding truncated proteins. Intronic PolyAdenylation regulates 20% of genes and is especially common in receptor tyrosine-kinase transcripts, generating soluble repressors. Here we report that human CD46, encoding a TransMembrane repressor of complement and T-cell co-stimulator, expresses multiple isoforms by alternative polyadenylation. We provide evidence for polyadenylation at several introns by RT-PCR of 5â² intronic fragments, and by increase in such isoforms via functional U1 knockdown. We mapped various Intronic PolyAdenylation Sites by 3â² Rapid Amplification of cDNA Ends (3â²RACE), which could generate soluble or membrane-bound but tail-less CD46. Intronic PolyAdenylation could add to the source of soluble CD46 isoforms in fluids and tissues, which increase in cancers and autoimmune syndromes. Furthermore, 3â²RACE identified three PolyAdenylation Sites within the last intron and exon, whose transcripts with shortened 3â²UTRs could support higher CD46 expression. Finally, 3â²RACE revealed that the CD46 Pseudogene only expresses short transcripts by early polyadenylation in intron 2. Overall, we report a wide variety of CD46 mRNA isoforms which could generate new protein isoforms, adding to the diverse physiological and pathological roles of CD46.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 625, 20 August 2017, Pages 21-30
Journal: Gene - Volume 625, 20 August 2017, Pages 21-30
نویسندگان
Phuong Thao Ly, Sze Jing Tang, Xavier Roca,