کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589664 | 1569809 | 2017 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Role of rs13117307 single nuclear polymorphism in the risk of uterine cervical cancer from Polish population and its impact on exocyst complex component 1 expression
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
Hardy-Weinbergb2mFIGOHMBSqRT-PCRM-MLVHRMSCCcDNA - cDNAcomplimentary DNA - DNA مجانیSybr Green I - Sybr سبز منχ2 - x2Expression - اصطلاحBeta-2-microglobulin - بتا-2-میکروگلوبولینHigh-resolution melting - ذوب با وضوح بالاCervical cancer - سرطان دهانه رحمNumber - عددInternational Federation of Gynecology and Obstetrics - فدراسیون بین المللی زنان و زایمانmajor histocompatibility complex - مجموعه سازگاری بافتی اصلیMHC - مجموعه سازگاری بافتی اصلیGenome-wide association studies - مطالعات مرتبط با ژنومGWAS - مطالعهٔ همخوانی سراسر ژنومodds ratio - نسبت شانس هاhydroxymethylbilane synthase - هیدروکسی متیلبیلن سنتازPolymorphism - پلی مورفیسمSingle nucleotide polymorphism - پلیمورفیسم تک نوکلئوتیدیSNP - چندریختی تک-نوکلئوتیدChi-square - چی مربعCervical squamous cell carcinoma - کارسینوم سلول سنگفرشی سرویکس
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Role of rs13117307 single nuclear polymorphism in the risk of uterine cervical cancer from Polish population and its impact on exocyst complex component 1 expression Role of rs13117307 single nuclear polymorphism in the risk of uterine cervical cancer from Polish population and its impact on exocyst complex component 1 expression](/preview/png/5589664.png)
چکیده انگلیسی
We evaluated the role of NM_001024924.1:c.1330 + 1646C > T (rs13117307) single nucleotide polymorphism (SNP), situated in the intronic region of exocyst complex component 1 (EXCO1), in the development and spreading of cervical squamous cell carcinoma (SCC). Utilizing high resolution melting curve analysis, we analyzed this polymorphism in patients with cervical SCC (n = 485) and controls (n = 509) in the Polish Caucasian population. Logistic regression analysis was used to adjust for age, parity, oral contraceptive use, tobacco smoking, and menopausal status. The influence of this polymorphism on the expression of EXCO1 was assessed by reverse transcription and real-time quantitative PCR analysis. For all patients with SCC, the p trend value calculated for rs13117307 was statistically significant (ptrend = 0.0158). The adjusted odds ratio (OR) for T/T vs. C/C was 1.434 (95 % CI 1.105-1.861, p = 0.007). We also found a significant contribution of rs13117307 to tumor stages III, IV and grade of differentiation G3. Other contributors are parity, oral contraceptive use, smoking, and women of postmenopausal age. We observed significant upregulation of EXCO1 transcript levels in the non-cancerous cervical tissues in carriers of the T/T vs. C/C (p = 0.016), as well as an increase in the EXCO1 transcript levels in the cervical SCC tissue in carriers of the T/T vs. C/C (p = 0.029) and for T/T vs C/T (p = 0.0032). The rs13117307 SNP variants may upregulate the transcription of EXCO1, as well as the risk of development and spreading of cervical SCC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 627, 5 September 2017, Pages 524-529
Journal: Gene - Volume 627, 5 September 2017, Pages 524-529
نویسندگان
Sebastian Åaźniak, Andrzej Roszak, Adam Balcerek, Å»aneta WareÅczak-Florczak, Edyta Prokop, Anna SowiÅska, Emianka Sotiri, Alexander Tsibulski, Stefan Sajdak, Pawel P. JagodziÅski,