کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589890 | 1569837 | 2017 | 34 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Anti-obesity effect of radix Angelica sinensis and candidate causative genes in transcriptome analyses of adipose tissues in high-fat diet-induced mice
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کلمات کلیدی
qPCRfragments per kilobase of transcript per million fragments mappedME1ApoA2PCK1FPKMMalic enzyme 1PNPLA3Radix Angelica sinensisHFDDEGsRASFDRTCMs - TCM هاApolipoprotein A-II - آپولیپوپروتئین A-IITranscriptome - ترانسکریپتومChinese herbal medicines - داروهای گیاهی چینیKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوhigh-fat diet - رژیم غذایی با چربی بالاRIN - رینRNA Integrity Number - شماره یکپارچه RNAphosphoenolpyruvate carboxykinase 1 - فسفوآنولپیروبات کربوکسیکیناز 1Obesity - مرض چاقیMice - موشfalse discovery rate - میزان کشف کاذبquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیAdipose - چربیCidea - کید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have previously reported that radix Angelica sinensis (RAS) suppressed body weight and altered the expression of the fat mass and obesity associated (FTO) gene in mice with high fat diet (HFD)-induced obesity. In the present study we performed RNA sequencing-mediated transcriptome analysis to elucidate the molecular mechanisms underlying the anti-obesogenic effects of RAS in mice. The results revealed that 36 differentially-expressed genes (DEGs) were identified in adipose tissues from the RAS supplementation group (DH) and control group (HC). These 36 DEGs were clustered into 297 functional gene ontology (GO) categories, among which several GO annotations and signaling pathways were associated with lipid homeostasis. Six out of the 36 DEGs were identified to be involved in lipid metabolism, with the APOA2 gene a potential anti-obesogenic influence. The expression pattern revealed by RNA-Seq was identical to the results of quantitative real-time PCR (qPCR). Therefore, RAS supplementation in HFD-induced obese mice was associated with an anti-obesogenic global transcriptomic response. This study provides insight into potential applications of RAS in obesity therapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 599, 30 January 2017, Pages 92-98
Journal: Gene - Volume 599, 30 January 2017, Pages 92-98
نویسندگان
Tao Zhong, Hao Zhang, Xiaoyue Duan, Jiangtao Hu, Linjie Wang, Li Li, Hongping Zhang, Lili Niu,