Biochemical and molecular genetic characterization of some species of family Malvaceae, Egypt

The aim of the present study intended mainly to investigate the interrelationships between the six studied taxa namely Abutilon theophrasti, Lavatera cretica, Hibiscus trionum, Hibiscus sabdariffa, Malva parviflora and Sida alba collected from ten different accessions in Egypt belonging to family Malvaceae. Biochemical studies include protein profile using SDS-PAGE technique and three isozymes (esterase, peroxidase and acid phosphatase). The electrophoretic analysis revealed the presence of eighteen bands of molecular weight ranging from 11.3 to 115.3 KD. The highest number of bands 15 was observed in H. sabdariffa (Hs1) collected from Menia el-Kamh district and the two accessions of M. parviflora where as the lowest number 11 bands were recorded in H. trionum collected from Talkha district. Four loci of peroxidase isozyme distinguished, three loci of acid phosphatase isozyme and two loci of esterase isozyme. In regarding to random amplified polymorphic DNA technique (RAPD), ten primers were used to differentiate between these accessions. Primer OPA-4 gave the highest percentage of polymorphism (100%), while primer OP-B6 produced the lowest percentage (50%).