کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5665230 | 1407738 | 2017 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Suppression of MUC5AC expression in human bronchial epithelial cells by interferon-γ Suppression of MUC5AC expression in human bronchial epithelial cells by interferon-γ](/preview/png/5665230.png)
BackgroundExcessive mucin secretion in the airway is an important feature of airway inflammatory diseases. MUC5AC expression is regulated by a variety of stimuli such as cytokines. Little is known about the role of interferon (IFN)-γ in MUC5AC expression in human bronchial epithelial cells.MethodsHuman pulmonary mucoepidermoid carcinoma cell line (NCI-H292) and normal human bronchial epithelial (NHBE) cells were used to assess the effects of IFN-γ on MUC5AC transcription.ResultsTransforming growth factor (TGF)-α and double-stranded RNA (polyI:C)-induced MUC5AC mRNA and protein expression was repressed by IFN-γ in a concentration-dependent manner. IFN-γ showed limited effects on TGF-α and polyI:C-induced activation of epidermal growth factor receptor (EGFR) and extracellular signal-regulated kinase (ERK). A chromatin immunoprecipitation assay indicated that Sp1 bound to its cognate sequence located on the MUC5AC promoter. The Sp1 inhibitor mithramycin A inhibited MUC5AC mRNA expression, implying a critical role for Sp1 in MUC5AC induction. Importantly, IFN-γ impeded Sp1 binding to the MUC5AC promoter.ConclusionsThese results suggest that IFN-γ represses MUC5AC expression, disturbing binding of Sp1 to its target sequences.
Journal: Allergology International - Volume 66, Issue 1, January 2017, Pages 75-82