کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5665232 1407738 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of sublingual immunotherapy in a murine asthma model sensitized by intranasal administration of house dust mite extracts
ترجمه فارسی عنوان
اثرات ایمونوتراپی زیر جلدی در یک مدل آسم چینی حساس شده توسط تزریق داخل بینی از عصاره گردو
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی ایمونولوژی، آلرژی و روماتولوژی
چکیده انگلیسی

BackgroundSublingual immunotherapy (SLIT) has received attention as a method for allergen immunotherapy. However, the mechanism of SLIT has not yet been fully investigated. Therefore, we evaluated the effects of SLIT in a murine asthma model, sensitized by intranasal administration of house dust mite (HDM) extracts.MethodsFemale BALB/c mice were intranasally exposed to HDM for either 3 or 5 weeks (5 consecutive days per week). Mice were administered either low-dose (0.5 mg/day) or high-dose (5 mg/day) sublingual HDM extracts for 2 weeks, followed by an additional week of intranasal exposure. Airway hyperresponsiveness (AHR), bronchoalveolar lavage fluid (BALF) cell count, cytokine levels in the BALF and lymph node cell culture supernatants, and allergen-specific antibodies were measured. Lung histology was also investigated.ResultsIn mice sensitized for 5 weeks, high-dose SLIT ameliorated AHR, airway eosinophilia and goblet cell metaplasia. In mice sensitized for 3 weeks, even low dose SLIT ameliorated AHR and airway eosinophilia. Th2 cytokine levels in culture supernatants of submandibular lymph node cells in high-dose SLIT mice decreased, whereas IL-10 levels increased. Total IgA in BALF increased in mice sensitized for 3 or 5 weeks, and high-dose SLIT also increased allergen-specific IgG2a in mice sensitized for 5 weeks.ConclusionsThese data suggest that earlier induction of SLIT in HDM-sensitized mice provides superior suppression of AHR and goblet cell metaplasia. The modulation of allergen specific IgG2a and local IgA might play a role in the amelioration of AHR and airway inflammation.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Allergology International - Volume 66, Issue 1, January 2017, Pages 89-96
نویسندگان
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