کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5665820 1407773 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid detection of high-risk Enterococcus faecium clones by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
ترجمه فارسی عنوان
تشخیص سریع کلونهای پرخطر انتروکوکوس فاکیمیم با استفاده از اسپکترومتر جرم زمان یونیزاسیون لیزر جذب ماتریکس
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
چکیده انگلیسی


- This is one of the first studies addressing the potential of MALDI-TOF MS in the identification of dominant E. faecium clones.
- Discrimination between isolates of high-risk clones and strains from community-based settings was obtained with >99% accuracy.
- Differentiation of STs belonging to different clonal lineages was accurately achieved in >95% of cases, while highly related STs were hardly distinguishable.

We aimed to explore the potential of matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for early identification of dominant Enterococcus faecium (Efm) clones involved in human infections. Well-characterized Efm isolates (n = 77), analyzed by pulsed-field gel electrophoresis and multilocus sequence typing(eBURST and BAPS [Bayesian analysis of population structure] algorithms), and belonging to different hospital (n = 53) and community (n = 24) phylogenomic groups, were tested. Mass spectra (Bruker) were analyzed by visual inspection and different chemometric tools. Discrimination between groups comprising isolates commonly found in hospitals (BAPS 2.1a, 3.3a1, 3.3a2) and community (BAPS 2.1b and 3.2) was achieved with >99% accuracy, while identification of sequence types belonging to different BAPS subgroups was associated with >95% correct predictions. Our work is a proof of concept with regard to the suitability of MALDI-TOF MS in the identification of high-risk Efm clones. Further studies including strains from a wider variety of clones and sources will strengthen the potential of the workflow here described.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 87, Issue 4, April 2017, Pages 299-307
نویسندگان
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