کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5665932 | 1407778 | 2017 | 5 صفحه PDF | دانلود رایگان |
- A multicenter evaluation of the RIDA® QUICK for detection of norovirus is presented.
- The RIDA® QUICK can be used to reliable confirm norovirus in stool samples.
- The RIDA® QUICK was able to detect norovirus variants that were missed by commercial PCR's.
- A negative result does not definitively exclude the presence of norovirus.
The updated RIDA® QUICK (N1402) immunochromatographic assay (R-Biopharm) for detection of norovirus was evaluated during a prospective, multicenter study using 771 stool samples from patients with gastroenteritis. Compared to real-time reverse transcriptase polymerase chain reaction (RT-rtPCR) as gold standard, the RIDA® QUICK had an overall sensitivity of 72.8% (91/125) and a specificity of 99.5% (640/643). Genotype analysis of the polymerase (ORF1) and capsid (ORF2) region of the genome indicated that the RIDA® QUICK assay could detect a broad range of genotypes including new variants (15 of 125 positive samples) which were detected by an in-house SYBR®Green RT-rtPCR, but not by the RIDA® GENE PCR PG1415 (R-Biopharm) and mostly not by the RIDA® GENE PCR PG1405 and the Xpert® Norovirus assay (Cepheid). The RIDA® QUICK can be used to reliably confirm norovirus in stool samples, but a negative result does not definitively exclude the presence of norovirus.
Journal: Diagnostic Microbiology and Infectious Disease - Volume 88, Issue 1, May 2017, Pages 31-35