کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5666898 1591736 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Global acquisition of genetic material from different bacteria into the staphylococcal cassette chromosome elements of a Staphylococcus epidermidis isolate
ترجمه فارسی عنوان
به دست آوردن جهانی مواد ژنتیکی از باکتری های مختلف به عناصر کروموزومی کاست استافیلوکوک استافیلوکوکوس اپیدرمیدیس جداسازی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
چکیده انگلیسی


- Staphylococcus epidermidis is a main reservoir of resistance and virulence genes.
- Potential assembly processes of three different SCC elements were demonstrated.
- Uncommon mobile genetic elements with different origins in SCC elements were identified.
- First report of the involvement of hsdS in genetic material exchange between staphylococci.

Staphylococcus epidermidis has been suggested as a main reservoir of methicillin resistance and virulence genes facilitating the evolution of Staphylococcus aureus as a successful pathogen. However, it remains a mystery where and how S. epidermidis obtains these numerous genes to serve as the reservoir. In this study, methicillin-resistant S. epidermidis isolate NW32 from a mastitic milk sample was sequenced and its staphylococcal cassette chromosome (SCC) elements were characterised. The SCC composite island covered 3.5% of the genome and consisted of three intact SCC elements carrying resistance genes against β-lactam antibiotics, several heavy metals and polyamines as well as genes for utilisation of sorbitol as a carbon source. Analysis of the postulated evolutionary route suggested that the three SCC elements were assembled from genetic material from various bacterial species (staphylococci, streptococci, salinicocci and Lysinibacillus) from three habitats (human, soil and cow) in different countries (Asia, North America, South America and Europe). We propose that the hsdS restriction-modification profile and the lack of CRISPR (clustered regularly interspaced short palindromic repeat) sequences in this bacterium may facilitate the genetic exchange of SCC elements among different staphylococcal species.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Antimicrobial Agents - Volume 50, Issue 4, October 2017, Pages 581-587
نویسندگان
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