کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5667372 | 1592038 | 2017 | 4 صفحه PDF | دانلود رایگان |
- The NDM-1, NDM-3 and NDM-5 âproducing bacteria were spread in China.
- NDM-5-produing S.typhimurium and NDM-3-produing E.coli were identified in China.
- The NDM-positive trains were resistant to nearly all of the antimicrobial drugs.
- blaNDM was mostly located on diverse plasmids with sizes from 30 to 670Â kb.
- An ongoing surveillance of their dissemination is essential to prevent their spread.
ObjectivesThe prevalence and dissemination of diverse NDM-producing bacteria in China was investigated.MethodsWe collected 1,162 isolates from 8 cities during December 2013 â¼Â May 2015 in China. The NDM-positive strains as well as the NDM genotypes in these sample were detected via Vitek 2 compact system (bioMérieux, France), 16S rRNA gene sequencing, PCR and an S1- pulsed-field gel electrophoresis assay and Southern blot hybridization. The horizontal-transfer capability of the blaNDM gene was assessed by filter mating by using a standard E.coli J53 azide-resistant strain as the recipient.ResultsThree genotypes (NDM-1, NDM-3 and NDM-5) of NDM-producing bacteria were identified, among which the NDM-1-positive isolates were the most frequent one. For the first time, we found NDM-5-produing S.typhimurium and NDM-3-produing E.coli in China. We also found that the NDM-positive (especially NDM-3 and NDM-5) strains were completely resistant to nearly all of the antimicrobial drugs utilized and blaNDM was mostly located on diverse plasmids with sizes ranging from 30 to 670 kb.ConclusionVarious species of bacteria especially the enteric pathogens with diverse NDM genotypes had spread in China. Hence, an ongoing surveillance of their dissemination is essential to prevent and control the spread of these organisms.
Journal: International Journal of Infectious Diseases - Volume 55, February 2017, Pages 92-95