کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5669337 1407957 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Tendon and ligament as novel cell sources for engineering the knee meniscus
ترجمه فارسی عنوان
تاندون و رباط ها به عنوان منابع سلولی جدید برای مهندسی منیسک زانو
کلمات کلیدی
مهندسی بافت، منابع سلول اولیه، فینیشر منیسک، فرآیند خودمراقبتی، عمل جراحی زانو،
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی ایمونولوژی، آلرژی و روماتولوژی
چکیده انگلیسی

SummaryObjectiveThe application of cell-based therapies in regenerative medicine is hindered by the difficulty of acquiring adequate numbers of competent cells. For the knee meniscus in particular, this may be solved by harvesting tissue from neighboring tendons and ligaments. In this study, we have investigated the potential of cells from tendon and ligament, as compared to meniscus cells, to engineer scaffold-free self-assembling fibrocartilage.MethodSelf-assembling meniscus-shaped constructs engineered from a co-culture of articular chondrocytes and either meniscus, tendon, or ligament cells were cultured for 4 weeks with TGF-β1 in serum-free media. After culture, constructs were assessed for their mechanical properties, histological staining, gross appearance, and biochemical composition including cross-link content. Correlations were performed to evaluate relationships between biochemical content and mechanical properties.ResultsIn terms of mechanical properties as well as biochemical content, constructs engineered using tenocytes and ligament fibrocytes were found to be equivalent or superior to constructs engineered using meniscus cells. Furthermore, cross-link content was found to be correlated with engineered tissue tensile properties.ConclusionTenocytes and ligament fibrocytes represent viable cell sources for engineering meniscus fibrocartilage using the self-assembling process. Due to greater cross-link content, fibrocartilage engineered with tenocytes and ligament fibrocytes may maintain greater tensile properties than fibrocartilage engineered with meniscus cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Osteoarthritis and Cartilage - Volume 24, Issue 12, December 2016, Pages 2126-2134
نویسندگان
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