کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5703940 | 1602557 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Non-invasive tryptophan fluorescence measurements as a novel method of grading cataract
ترجمه فارسی عنوان
اندازه گیری های فلورسنت تریپتوفان غیر تهاجمی به عنوان یک روش جدید درجه بندی آب مروارید
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کلمات کلیدی
PMTNFKN-formylkynurenineFLACSScheimpflugARGPDLSPSCPBSCataract - آب مرواریدArgpyrimidine - ارگپیریمیدینTryptophan - تریپتوفانGrading - درجه بندیNon-invasive - غیر تهاجمیPhosphate buffered saline - فسفات بافر شورFluorescence - فلورسنسphotomultiplier tube - لوله فوتومولتیپلیرDynamic Light Scattering - پراکندگی نور دینامیکیposterior subcapsular cataract - کاتاراکت زیر کپسول خلفی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی و میکروب شناسی (عمومی)
چکیده انگلیسی
Development of non-invasive treatments for cataract calls for a sensitive diagnostic assay. We conducted a study to test whether the ratio of folded tryptophan to non-tryptophan fluorescence emission (F-factor) may be used for grading cataracts in human lenses. The F-factor was measured on aspirated lens material from eyes undergoing femtosecond laser assisted cataract surgery (FLACS) and was compared to a preoperative optical grading of cataract using Scheimpflug imaging. The preoperative optical grading allocated the cataracts to 1 of 4 categories according to the density of the cataract. All cataracts were age-related. Lens material from 16 eyes of 14 patients was included in the study. Cataracts were preoperatively graded in categories 1, 2 and 3. No lenses were category 4. For nuclear cataracts mean values of F-factor were 52.9 (SD 12.2), 61.7 (SD 5.3) and 75.7 (SD 8.9) for categories 1, 2 and 3 respectively. Linear regression on F-factor as a function of preoperative grading category showed increasing values of F-factor with increasing preoperative grading category, R2Â =Â 0.515. Our experiment showed that preoperative optical grading of cataracts by Scheimpflug imaging may correlate to measures of tryptophan and non-tryptophan fluorescence in human lenses. Based on our results we find that measuring the ratio between tryptophan- and non-tryptophan fluorescence may be a future tool for grading cataracts, but further research is needed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Eye Research - Volume 165, December 2017, Pages 59-64
Journal: Experimental Eye Research - Volume 165, December 2017, Pages 59-64
نویسندگان
Jesper Høiberg Erichsen, Aurore Mensah, Line Kessel,