کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5704080 | 1602569 | 2016 | 52 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Alteration in cellular turnover and progenitor cell population in lacrimal glands from thrombospondin 1â/â mice, a model of dry eye
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی و میکروب شناسی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The purpose of this study was to investigate the changes that occur in the lacrimal glands (LGs) in female thrombospondin 1 knockout (TSP1â/â) mice, a mouse model of the autoimmune disease Sjogren's syndrome. The LGs of 4, 12, and 24 week-old female TSP1â/â and C57BL/6J (wild type, WT) mice were used. qPCR was performed to measure cytokine expression. To study the architecture, LG sections were stained with hematoxylin and eosin. Cell proliferation was measured using bromo-deoxyuridine and immunohistochemistry. Amount of CD47 and stem cell markers was analyzed by western blot analysis and location by immunofluorescence microscopy. Expression of stem cell transcription factors was performed using Mouse Stem Cell Transcription Factors RT2 Profiler PCR Array. Cytokine levels significantly increased in LGs of 24 week-old TSP1â/â mice while morphological changes were detected at 12 weeks. Proliferation was decreased in 12 week-old TSP1â/â mice. Three transcription factors were overexpressed and eleven underexpressed in TSP1â/â compared to WT LGs. The amount of CD47, Musashi1, and Sox2 was decreased while the amount of ABCG2 was increased in 12 week-old TSP1â/â mice. We conclude that TSP1 is necessary for maintaining normal LG homeostasis. Absence of TSP1 alters cytokine levels and stem cell transcription factors, LG cellular architecture, decreases cell proliferation, and alters amount of stem cell markers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Eye Research - Volume 153, December 2016, Pages 27-41
Journal: Experimental Eye Research - Volume 153, December 2016, Pages 27-41
نویسندگان
Marie A. Shatos, Robin R. Hodges, Masahiro Morinaga, David E. McNay, Rakibul Islam, Sumit Bhattacharya, Dayu Li, Bruce Turpie, Helen P. Makarenkova, Sharmila Masli, Tor P. Utheim, Darlene A. Dartt,