کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5734061 1612514 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pediatric/Congenital/DevelopmentalIn-bag enzymatic splenic digestion: a novel alternative to manual morcellation?
ترجمه فارسی عنوان
هضم آلزایمر کودکان / مادرزادی / رشد در داخل کیسه: یک جایگزین جدید برای تغییرات دستی؟
کلمات کلیدی
هضم بافت آنزیمی، برش زیستی، کلاژناز، طحال، اسپلنکتومی حداقل مهاجم، بازیابی جایگزین نمونه،
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی عمل جراحی
چکیده انگلیسی

BackgroundContained in-bag spleen morcellation is a conventional extraction technique for safe spleen removal during laparoscopic splenectomy. Existing data for the use of in-bag enzymatic splenic digestion as an alternative to morcellation are lacking. This proof-of-concept study sought to evaluate the effectiveness of single and combinatorial enzyme digestion of murine spleens.Materials and methodsMurine spleens were digested with collagenase alone or with combinations of commercially available enzymes (collagenase, elastase, hyaluronidase, neutral protease) to determine their degradation effect. The primary end point was the percentage of mass reduction at 15 and 30 min.ResultsFor collagenase alone (n = 15), the mean reduction in mass was 14 ± 10% (range: 2%-31%) at 15 min and 30 ± 25% (range: 7%-100%) at 30 min. Using combinatorial dissolution with collagenase, hyaluronidase, and elastase (n = 8), the mean reduction in mass was 27 ± 16% (range: 6%-42%) at 15 min and 48 ± 27% (range: 3%-100%) at 30 min. Injecting the enzyme solution into whole spleens (n = 9) yielded a mean reduction in mass of 22 ± 13% (range: 9%-42%) at 15 min and 55 ± 31% (range: 9%-100%) at 30 min; mean reduction was 9 ± 13% (range: 0%-39%) at 15 min and 23 ± 13% (range: 3%-53%) with no injection (n = 12).ConclusionsWe provide the first demonstration of successful enzymatic murine spleen digestion as an alternative method for in-bag spleen removal during laparoscopic splenectomy. However, the significant cost and quantities of commercial enzyme required for clinical application dampens the enthusiasm for this novel approach.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Surgical Research - Volume 218, October 2017, Pages 209-216
نویسندگان
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