کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5736581 1613788 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Deep brain stimulation of the anterior nucleus of the thalamus reverses the gene expression of cytokines and their receptors as well as neuronal degeneration in epileptic rats
ترجمه فارسی عنوان
تحریک مغزی عمیق از هسته قدام تالاموس، بیان ژن سیتوکین ها و گیرنده های آنها و نیز دژنرسانس عصبی در موش های صرعی را معکوس می کند
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


- ANT-DBS could suppress cytokines and their receptors expressions in epileptic rat.
- ANT-DBS was capable of reducing neuronal structural degeneration in epileptic rat.
- ANT-DBS could be effective if performed in early stage of epilepsy.

BackgroundDeep brain stimulation of the anterior nucleus of the thalamus (ANT-DBS) is effective in seizure control. However, the mechanisms remain unclear.MethodsSixty-four rats were randomly assigned to the control group, the kainic acid (KA) group, the sham-DBS group and the DBS group. Video-electroencephalogram (EEG) was used to monitor seizures. Quantitative real time PCR (qPCR) was applied for detecting interleukin-1 beta (IL-1β), IL-1 receptor (IL-1R), IL-6, IL-6 receptor (IL-6R), gp130, tumor necrosis factor-alpha (TNF-α), TNF-receptor 1 (TNF-R1) and TNF-receptor 2 (TNF-R2) expression 12 h after the establishment of an epileptic model. The neuronal structural degeneration in the hippocampus was evaluated with transmission electron microscopy (TEM) at this same time point.ResultsThe seizure frequency was 48.6% lower in the DBS group compared with the sham-DBS group (P < 0.01). The expression of IL-1β, IL-1R, IL-6, IL-6R, gp130, TNF-α and TNF-R1 was elevated in both the KA and the sham group compared with the control group (all Ps < 0.01). Additionally, ANT-DBS was able to reverse this gene expression pattern in the DBS group compared with the sham-DBS group (all Ps < 0.01). There was no significant difference in TNF-R2 expression among the four groups. The neuronal structural degeneration in the KA group and the sham-DBS group was more severe than that in the control group (injury scores, all Ps < 0.01). ANT-DBS was also capable of relieving the degeneration compared with the sham-DBS group (injury score, P < 0.01).ConclusionsThis study demonstrated that ANT-DBS can reduce seizure frequency in the early stage in epileptic rats as well as relieve the pro-inflammatory state and neuronal injury, which may be one of the most effective mechanisms of ANT-DBS against epileptogenesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1657, 15 February 2017, Pages 304-311
نویسندگان
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