Regular paperA novel device for continuous long-term electroencephalogram recording and drug administration in mice with a nice, powerful and sophisticated wired system

- We constructed a sophisticated device for long term EEG recording and drug infusion in moving mice.
- Continuous EEG recording was recorded for up to 63 days.
- Continuous drug infusion with EEG recording was succeeded for up to 22 days.
- The device will be useful to develop new antiepileptic drugs.

BackgroundTo elucidate mechanisms of epileptogenesis and epileptic maturation, and to develop new AEDs, it is indispensable to administer various drugs and to examine their effects on EEG over a long period of observation.New MethodWe constructed a device for the continuous measurement of electroencephalography (EEG) and the infusion of anti-epileptic drugs over a prolonged period of time in moving mice. The system includes a slip ring and a swivel to prevent twisting of the recording cable and infusion tube, respectively. We introduced three arms, ball bearing, and stabilizing frame to rotate the slip ring and swivel with only a small applied force, and to facilitate the start of rotation of the slip ring and the swivel.ResultsContinuous EEG recording was successfully performed for up to 63 days in 99 mice, for a total of 1872 days of EEG data. Continuous drug infusion with continuous EEG recording was successfully performed for up to 22 days.Comparison with Existing Method(s)Our system is superior to current system in continuous drug delivery during long-term EEG recording in moving mouse.ConclusionsOur device will be quite useful for long-term EEG recording and drug application in moving mice.