Alterations of motor cortical microcircuit in a depressive-like mouse model produced by light deprivation

- Light deprivation could produce a depressive behavioral phenotype in mice.
- The depressive behavioral phenotype was characterized by increased immobility and decreased locomotor activity.
- Light deprivation decreased burst firing neurons, suppressed neural excitability and reduced neural morphological complexity.
- Light deprivation decreased the probability of single connections formed by L5PCs.
- Light deprivation increased the synaptic strength and transmitter release probability of single L5PC connections.

Depression is one of the most prevalent and life-threatening forms of mental illness. The heavy social burden imposed by this disorder calls for a better understanding of its pathogenesis. Light deficiency is an important factor potentially leading to depression. However, how the light deficiency affects neural microcircuit underlying depression remains largely unknown. This study investigated the properties of morphology, electrophysiology, and synaptology of layer V pyramidal cells (L5PCs) in the motor cortex of a mouse model with depressive behavioral phenotype that was produced by light deprivation (LD). The depressive behavioral phenotype was characterized by increased immobility and decreased locomotor activity in behavioral tests. LD decreased burst firing neurons and suppressed the intrinsic excitability of L5PCs, and also reduced the neuronal morphological complexity as evidenced by simplified basal and apical dendrites. Moreover, LD reduced the synaptic connecting probability of L5PCs. These alterations of the simplified morphology, the suppressed excitability, and the reduced connecting probability of L5PCs together could well explain the depression-like behaviors of the mouse model. However, it was surprising to find that the excitatory postsynaptic potentials (EPSPs) of single L5PC connections were significantly enhanced and the paired pulse ratio (EPSP2/EPSP1) was significantly increased. These synaptological results indicate that the absolute synaptic strength of single L5PC connections was enhanced and the transmitter release probability was increased although the connections between L5PCs became sparse. Therefore, a compensation mechanism accompanied the negative changes that were consistent with the depressive behavioral phenotype. Our findings from the motor cortex of depression-like behavior mice may underlie the neural microcircuit mechanism of depression, providing insights into the pathogenesis of depression at a level of single neurons and synaptic connections.

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