کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5739010 | 1615268 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Roles of 5-HT1A receptor in the expression of AMPA receptor and BDNF in developing mouse cortical neurons
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Roles of 5-HT1A receptor in the expression of AMPA receptor and BDNF in developing mouse cortical neurons Roles of 5-HT1A receptor in the expression of AMPA receptor and BDNF in developing mouse cortical neurons](/preview/png/5739010.png)
چکیده انگلیسی
The possible interactions between serotonergic and glutamatergic systems during neural development and under the pathogenesis of depression remain unclear. We now investigated roles of 5-HT1A receptor in the mRNA expression of AMPA receptor subunits (GluR1 and GluR2) and brain-derived neurotrophic factor (BDNF) using primary culture of cerebral cortex of mouse embryos. Neurons at embryonic day 18 were cultured for 3Â days or 14Â days and then treated with 5-HT1A receptor agonist (8-OH-DPAT) for 3Â h or 24Â h. In neurons cultured for 3 days, 8-OH-DPAT treatment for both 3Â h and 24Â h increased the mRNA levels of BDNF and GluR1, but not GluR2. In neurons cultured for 14 days, however, 8-OH-DPAT had no effects on these mRNA levels. Next, we examined in vivo roles of 5-HT1A receptor by administration of 8-OH-DPAT to newborn mice. Twenty-four hours after the oral administration of 8-OH-DPAT, the mRNA expression of BDNF was decreased in the frontal cortex, but had no effects on the mRNA expression of GluR1 and GluR2. Taken together, the present study suggests that 5-HT1A receptor activation modulates mRNA expression of AMPA receptor subunit and BDNF in cortical neurons, and the effects are different between in vitro and in vivo.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Research - Volume 115, February 2017, Pages 13-20
Journal: Neuroscience Research - Volume 115, February 2017, Pages 13-20
نویسندگان
Yuko Yoshimura, Chihiro Ishikawa, Haruki Kasegai, Tomoyuki Masuda, Masaaki Yoshikawa, Takashi Shiga,