کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5740663 1616526 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A survey of oenophages during wine making reveals a novel group with unusual genomic characteristics
ترجمه فارسی عنوان
یک بررسی از عفونت ها در طول تولید شراب نشان می دهد گروه جدید با ویژگی های ژنومی غیر معمول است
کلمات کلیدی
باکتریوفاژ، تنوع ساخت شراب، باید، اونوکوکوس اوین، تخمیر مالاولیک،
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی


- We isolated 71 bacteriophages infecting Oenococcus oeni from must and MLF samples.
- Some of the newly isolated phages form a novel group with unusual genomic characteristics.
- Development of novel signature genes for assessing oenophage diversity is required.

Oenophages have so far been mostly isolated from red wines under malolactic fermentation (MLF), and correspond to temperate or ex-temperate phages of Oenococcus oeni. Their genomes are clustered into 4 integrase gene sequence groups, which are also related to the chromosomal integration site. Our aims were to survey the occurrence of oenophages in a broader and more diverse collection of samples than those previously explored. Active phages were isolated from 33 out of 166 samples, which mostly originated from must and MLF. Seventy one phage lysates were produced and 30% were assigned to a novel group with unusual genomic characteristics, called unk. All unk members produced similar RAPD and DNA restriction patterns, were negative by PCR for the signature sequences previously identified in the integrase and endolysin genes of oenophages, and lacked any BamHI restriction site in their genome. The data support that development of additional and novel signature genes for assessing oenophage diversity is now required.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Food Microbiology - Volume 257, 18 September 2017, Pages 138-147
نویسندگان
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