Full Length ArticleParasitological and transcriptomic comparison of Strongyloides ratti infections in natural and in suboptimal permissive hosts

- S. ratti infect gerbils less efficiently than rats but survive longer in this host.
- S. ratti in gerbils reproduce well but produce a high proportion of males.
- Only few genes are differentially expressed between S. ratti in rats and in gerbils.
- Several astacins and acetylcholinesterases are differentially expressed.
- Gerbils are good laboratory hosts for the long-term maintenance of S. ratti.

The nematode genus Strongyloides consists of fairly species-specific small intestinal parasites of various vertebrates, among them the human pathogen S. stercoralis. Between the parthenogenetic parasitic generations these worms can also form single facultative sexual free-living generations. In addition to their primary hosts, several species can also live more or less well in other permissive hosts, which are sometimes not very closely related with the normal host. For example, S. stercoralis can also infect dogs and non-human primates.Here we compare the infection and reproductive success over time and the gene expression profiles as determined by quantitative sequencing of S. ratti parasitizing in its natural host rat and in the permissive host gerbil. We show that in gerbils fewer infective larvae successfully establish in the host, but those that do accomplish this survive and reproduce for longer and produced a higher proportion of males during the first two month of infection. Globally, the gene expression profiles in the two hosts are very similar. Among the relatively few differentially expressed genes, astacin-like and acetylcholinesterase genes are prominently represented. In the future it will be interesting to see if these changes in the suboptimal host are indeed ecologically sensible responses to the different host.

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