کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
575781 1453059 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Toxicity of polyhydroxylated fullerene to mitochondria
ترجمه فارسی عنوان
سمیت فولرین پلی هیدروکسیل شده به میتوکندریا
کلمات کلیدی
کلرید پلی هیدروکسیل شده، میتوکندری جدا شده، سیالیت غشای میتوکندری، انتقال نفوذپذیری میتوکندری،
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بهداشت و امنیت شیمی
چکیده انگلیسی
Mitochondrial dysfunction is considered as a crucial mechanism of nanomaterial toxicity. Herein, we investigated the effects of polyhydroxylated fullerene (C60(OH)44, fullerenol), a model carbon-based nanomaterial with high water solubility, on isolated mitochondria. Our study demonstrated that fullerenol enhanced the permeabilization of mitochondrial inner membrane to H+ and K+ and induced mitochondrial permeability transition (MPT). The fullerenol-induced swelling was dose-dependent and could be effectively inhibited by MPT inhibitors such as cyclosporin A (CsA), adenosine diphosphate (ADP), ruthenium red (RR) and ethylenediaminetetraacetic acid (EDTA). After treating the mitochondria with fullerenol, the mitochondrial membrane potential (MMP) was found collapsed in a concentration-independent manner. The fluorescence anisotropy of hematoporphyrin (HP) changed significantly with the addition of fullerenol, while that of 1,6-diphenyl-hexatriene (DPH) changed slightly. Moreover, a decrease of respiration state 3 and increase of respiration state 4 were observed when mitochondria were energized with complex II substrate succinate. The results of transmission electron microscopy (TEM) provided direct evidence that fullerenol damaged the mitochondrial ultrastructure. The investigations can provide comprehensive information to elucidate the possible toxic mechanism of fullerenols at subcellular level.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Hazardous Materials - Volume 301, 15 January 2016, Pages 119-126
نویسندگان
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