کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5762284 | 1624813 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bioactivity of essential oils in the control of Alternaria alternata in dragon fruit (Hylocereus undatus Haw.)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
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چکیده انگلیسی
This study isolated and identified the fungus that causes postharvest disease in dragon fruit (Hylocereus undatus Haw.). The in vitro and in vivo antifungal activity of some essential oils were evaluated against the fungus. Morphophysiological and molecular identification confirmed the fungus was Alternaria alternata. The essential oils of Cinnamomum zeylanicum, Cymbopogon flexuosus, Eucalyptus globulus, Eugenia caryophyllus, and Rosmarinus officinalis were evaluated by the microdilution broth technique, disc diffusion, scanning electron microscopy, and fluorescence microscopy. Evaluation of the composition of the essential oils by gas chromatography/mass spectrometry revealed substantial amounts of eugenol as a major constituent of E. caryophyllus and C. zeylanicum (90.50% and 80.70%, respectively). The other essential oils of R. officinalis contained α-pinene (24.5%) and camphor (22.0%) as major components. E. globulus contained 1,8-cineole (78.9%). C. flexuosus contained neral (35.1%) and geranial (42.6%). C. zeylanicum and E. caryophyllus were the most active against the isolated fungi at minimum inhibitory concentrations of 250 and 500 μg/ml, respectively, causing morphological changes in the hyphae. The in vivo assay indicated that the fruits that were treated with E. caryophyllus at concentrations of 500 and 1000 μg/ml exhibited a 31% reduction of mycelial growth compared with the control. These results suggest that the essential oils of C. zeylanicum and E. caryophyllus are active against A. alternata both in vitro and in vivo, which may be promising for control of the microorganism.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Industrial Crops and Products - Volume 97, March 2017, Pages 101-109
Journal: Industrial Crops and Products - Volume 97, March 2017, Pages 101-109
نویسندگان
Juliana Cristina Castro, Eliana Harue Endo, Marina Roberta de Souza, Erica Benassi Zanqueta, Julio Cesar Polonio, João Alencar Pamphile, Tânia Ueda-Nakamura, Celso Vataru Nakamura, Benedito Prado Dias Filho, BenÃcio Alves de Abreu Filho,