کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5763017 | 1625148 | 2017 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A polyphenol-enriched fraction of Cyclopia intermedia decreases lipid content in 3T3-L1 adipocytes and reduces body weight gain of obese db/db mice
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A polyphenol-enriched fraction of Cyclopia intermedia decreases lipid content in 3T3-L1 adipocytes and reduces body weight gain of obese db/db mice A polyphenol-enriched fraction of Cyclopia intermedia decreases lipid content in 3T3-L1 adipocytes and reduces body weight gain of obese db/db mice](/preview/png/5763017.png)
چکیده انگلیسی
Extracts of Cyclopia species, indigenous South African fynbos plants used for the production of honeybush tea, have potential as anti-obesity nutraceutical ingredients. Previously, we demonstrated that aqueous extracts of C. maculata and C. subternata exhibited anti-obesity effects in 3T3-L1 adipocytes. In this study, we further explored these anti-obesity effects of C. maculata and C. subternata as well as C. intermedia for the first time. Extracts were prepared using a 40% methanol-water mixture (40% MeOH) in order to enhance the polyphenolic content of the extracts. Moreover, these extracts were separated into aqueous and organic fractions using liquid-liquid partitioning with n-butanol and water to further enrich the polyphenol content of the organic fractions. Extracts of all three Cyclopia species decreased the lipid content in 3T3-L1 adipocytes, although differences in bioactivity of their aqueous and organic fractions were observed. The organic fraction of C. intermedia was further investigated. This fraction dose-dependently decreased the lipid content in 3T3-L1 adipocytes without affecting cell viability, while increasing mRNA expression of HSL (1.57-fold, P < 0.05), SIRT1 (1.5-fold, P = 0.07), UCP3 (1.5-fold, P < 0.05) and PPARγ (1.29-fold, P < 0.05). Daily treatment of obese db/db mice with 351.5 mg/kg bodyweight of the organic C. intermedia fraction for 28 days decreased bodyweight gain by 21% (P < 0.05) without any effect on food or water consumption. The organic fraction was enriched in phenolic content relative to the extract with neoponcirin, a flavanone not previously identified in Cyclopia species, mangiferin, isomangiferin and hesperidin comprising 17.37% of the organic fraction of C. intermedia compared to 4.96% of its “large scale” prepared 40% MeOH extract. Their specific roles as anti-obesity agents in these models needs to be studied to guide product development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: South African Journal of Botany - Volume 110, May 2017, Pages 216-229
Journal: South African Journal of Botany - Volume 110, May 2017, Pages 216-229
نویسندگان
B.U. Jack, C.J. Malherbe, B. Huisamen, K. Gabuza, S. Mazibuko-Mbeje, A.E. Schulze, E. Joubert, C.J.F. Muller, J. Louw, C. Pheiffer,