کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5763170 1625146 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Genetic fidelity assessment in plants of Sechium edule regenerated via organogenesis
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Genetic fidelity assessment in plants of Sechium edule regenerated via organogenesis
چکیده انگلیسی
The aim of this study was to develop an efficient protocol for the in vitro regeneration of chayote (Sechium edule), an important Mexican crop and the evaluation of the genetic fidelity. This species produces recalcitrant seeds, which complicates germplasm storage and makes in vitro conservation a desirable alternative. Proliferation of axillary shoots was induced from axenic nodal segments obtained from in vitro germinated seedlings. Nodal segments were cultured on MS medium supplemented with 0.1 mg/L 6-benzylaminopurine (BA), producing 8.0 ± 0.4 shoots per explant with a regeneration frequency of 92%. Plant regeneration by organogenesis was optimally achieved when leaf and petiole explants were cultured on MS medium supplemented with 0.1 mg/L BA and 0.05 mg/L gibberellic acid (GA3), obtaining an average of 5.3 ± 1.9 shoots per explant. All of the regenerated shoots originated directly from petioles without previous formation of callus. Adventitious shoots were successfully rooted in MS medium without growth regulators after 2 weeks. Since somaclonal variation is a potential hindrance to the in vitro propagation of plants, genetic stability was evaluated through RAMP markers using 9 combinations of RAPD and ISSR primers. The amplification products obtained from the regenerated plants were electrophoresed and showed banding patterns similar to that of the mother plant, demonstrating the homogeneity of the individuals obtained through the regeneration protocols reported here.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: South African Journal of Botany - Volume 112, September 2017, Pages 118-122
نویسندگان
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