Antioxidant and hepatoprotective effects of different ethanol concentrations in extraction from leaves of Sasa quelpaertensis Nakai

Biological ability and optimal extraction yield of Sasa quelpaertensis Nakai leaf extract with different ethanol (EtOH) concentrations (20, 40, 60, 80, and 100%) and distilled water were determined. The 60% EtOH extract archived the highest extraction yield and exhibited the highest rates of DPPH and ABTS radical scavenging, reducing power, and tyrosinase inhibitory activity. Also, when the various concentrations of EtOH (0-1600 mM) were applied, significant loss was observed at 400 mM and above. However, the 60% EtOH extract of S. quelpaertensis Nakai leaves induced cell viability by 7% and 9% in 400 and 800 μg/ml concentrations, respectively. Moreover, for the combination of 60% EtOH extract from S. quelpaertensis Nakai leaves (0-800 μg/ml) and 400 mM EtOH, a 6.3% increase of viability was observed at 800 μg/ml as compared to no sample treatment. These results indicate that S. quelpaertensis Nakai leaves could be good candidate as safe antioxidants and tyrosinase inhibitor.