کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5764224 | 1625917 | 2017 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Modifications of the soluble proteome of a mediterranean strain of the invasive neurotoxic dinoflagellate Alexandrium catenella under metal stress conditions
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کلمات کلیدی
FNRRBPHABNCBIDTTHspNADPHIEFG3PDHAPPSP2-DEPCPBiP - BIPFerredoxin-NADP+ reductase - Feredoxin-NADP + ردوکتازIPG - GPAimmobilized pH gradient - gradient pH immobilizedLC–MS/MS - LC-MS / MSMS/MS - MS / MStwo-dimensional gel electrophoresis - الکتروفورز ژل دو بعدیisoelectric focusing - تمرکز ذره ای الکتریکیdihydroxyacetone phosphate - دی هیدروکسی استون فسفاتdithiothreitol - دیتیوتریتولCAM - ساخت به کمک کامپیوترHarmful algal bloom - شکوفه جلبکMass spectrometry - طیف سنجی جرمیTandem mass spectrometry - طیف سنجی جرمی پشت سر هم یا متوالیNational Centre for Biotechnology Information - مرکز ملی اطلاعات بیوتکنولوژیParalytic shellfish poisoning - مسمومیت ناشی از پوسته پوسته پوسته شدنIsoelectric point - نقطه ایزوالکتریکnicotinamide adenine dinucleotide phosphate - نیکوتین آمید adenine dinucleotide phosphateMolecular weight - وزن مولکولیBinding immunoglobulin Protein - پروتئین ایمونوگلوبولین BindingHeat shock protein - پروتئین شوک حرارتCHAPS - چاپسcalr - کالرCalmodulin - کالمودولینliquid chromatography coupled to tandem mass spectrometry - کروماتوگرافی مایع همراه با طیف سنجی جرمی دو طرفهcalreticulin - کلرتی کولینglyceraldehyde 3-phosphate - گلیسرولیدید 3-فسفات
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The soluble proteome of the mediterranean strain ACT03 of the invasive neurotoxic dinoflagellate Alexandrium catenella exposed to lead or zinc at 6, 12 or 18 μM (total concentrations), or under control conditions, was characterized by two-dimensional gel electrophoresis (2-DE). Zinc reduced (P < 0.05) the total number of protein spots (â41%, â52% and â60%, at 6, 12 or 18 μM, respectively). Besides, most of the proteins constituting the soluble proteome were down-regulated in response to lead or zinc stresses. These proteins were involved mainly in photosynthesis (20-37% for lead; 36-50% for zinc) (ribulose-1,5-bisphosphate carboxylase/oxygenase: RUBISCO; ferredoxin-NADP+ reductase: FNR; peridinin-chlorophyll a-protein: PCP), and in the oxidative stress response (29-34% for lead; 17-36% for zinc) (superoxide dismutase: SOD; proteasome α/β subunits). These negative effects could be partly compensated by the up-regulation of specific proteins such as ATP-synthase β subunit (+16.3 fold after exposure to lead at 12 μM). Indeed, an increase in the abundance of ATP-synthase could enrich the ATP pool and provide more energy available for the cells to survive under metal stress, and make the ATP-synthase transport of metal cations out of the cells more efficient. Finally, this study shows that exposure to lead or zinc have a harmful effect on the soluble proteome of A. catenella ACT03, but also suggests the existence of an adaptative proteomic response to metal stresses, which could contribute to maintaining the development of this dinoflagellate in trace metal-contaminated ecosystems.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquatic Toxicology - Volume 188, July 2017, Pages 80-91
Journal: Aquatic Toxicology - Volume 188, July 2017, Pages 80-91
نویسندگان
Natacha Jean, Estelle Dumont, Faouzi Herzi, Thierry Balliau, Mohamed Laabir, Estelle Masseret, Stéphane Mounier,