کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
576740 1453087 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hexavalent chromium damages chamomile plants by alteration of antioxidants and its uptake is prevented by calcium
ترجمه فارسی عنوان
کروم شش رنگه با گیاهان بابونه توسط تغییر آنتی اکسیدان ها و جذب آن توسط کلسیم جلوگیری می شود
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بهداشت و امنیت شیمی
چکیده انگلیسی
Toxicity of low (3 μM) and high (60 and 120 μM) concentrations of hexavalent chromium/Cr(VI) in chamomile plants was studied. Fluorescence staining confirmed reduction of Cr(VI) to Cr(III). Cr was mainly accumulated in the roots with translocation factor <0.007. Notwithstanding this, both shoots and roots revealed increase in oxidative stress and depletion of glutathione, total thiols, ascorbic acid and activities of glutathione reductase and partially ascorbate peroxidase mainly at 120 μM Cr. Though some protective mechanisms were detected (elevation of nitric oxide, enhancement of GPX activity and increase in phenols and lignin), this was not sufficient to counteract the oxidative damage. Consequently, soluble proteins, tissue water content and biomass production were considerably depleted. Surprising increase in some mineral nutrients in roots (Ca, Fe, Zn and Cu) was also detected. Subsequent experiment confirmed that exogenous calcium suppressed oxidative symptoms and Cr uptake but growth of chamomile seedlings was not improved. Alteration of naturally present reductants could be a reason for Cr(III) signal detected using specific fluorescence reagent: in vitro assay confirmed disappearance of ascorbic acid in equimolar mixture with dichromate (>96% at pH 4 and 7) while such response of glutathione was substantially less visible.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Hazardous Materials - Volume 273, 30 May 2014, Pages 110-117
نویسندگان
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