کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5768280 1628456 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Study on external factors affecting egg yolk plasma by asymmetrical flow field-flow fractionation
ترجمه فارسی عنوان
بررسی عوامل خارجی که بر پلاسمای تخم مرغی تخم مرغ تاثیر می گذارند با تقسیم بندی میدان جریان نامتقارن
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی


- AF4-UV-MALS-QELS was applied for analysis of egg yolk plasma.
- The effect of external factors on the egg yolk plasma was investigated.
- The conformation of LDL aggregates was studied.
- The aggregation mechanism of LDL was discussed.

In this work, the ability of asymmetrical flow field-flow fractionation (AF4) coupled with UV-vis, multiangle light scattering (MALS), and quasi-elastic light scattering (QELS) (AF4-UV-MALS-QELS) for monitoring aggregation of components of egg yolk plasma was evaluated. The effect of external factors (i.e., pH, storage conditions, freezing and heat treatments) on the egg yolk plasma was studied. The results reveal that the aggregation mechanism of components of egg yolk plasma during heat and freezing treatment is different. The results suggest that the low density lipoproteins (LDLs) in egg yolk plasma undergo a 'clusters-fusion-gel' process under heat treatment at pH 10. The alkaline conditions promote the formation of LDL aggregates. Also, storage conditions play a role in the formation of LDL aggregates. It was found that the hen eggs stored for 7 days at room temperature contain less aggregate than those stored at 4 °C for the same period. The combination of AF4 with online MALS-QELS provided conformational information in terms of the shape and size distribution of LDL aggregates. AF4-UV-MALS-QELS was proved to be a rapid and gentle method for the separation and characterization of egg yolk plasma aggregates.

178

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Research International - Volume 94, April 2017, Pages 13-19
نویسندگان
, , , , , , , , , ,