A high-throughput multiplex tandem PCR assay for the screening of genetically modified maize

- A high-throughput screening system was developed for detection of GM maize.
- The system utilized multiplex tandem PCR for 14 targets detection.
- The method allowed for amplification of less to 0.001 ng of the template DNA.
- This method contributes to detecting unauthorized GMOs released into the markets.

Genetically modified organisms (GMO) are increasingly gaining acceptance, which has led to an increasing demand for high-throughput methods for their detection. This study describes development of a high-throughput and low-cost multiplex tandem PCR (MT-PCR) assay for the screening of transgenic maize of 14 targets, containing 7 genetically modified (GM) common screening elements, 6 transgenic maize events and a maize reference gene, using SYBR Green I for quantification. The 14-target high-throughput multiplex tandem PCR assay successfully amplified products from single-event samples with a GM maize DNA amount less to 0.001 ng, and displayed high specificity. This assay can efficiently be used for screening GM maize in foods and feeds.